I haven't done this myself, but I've heard about people doing it without trouble.
You specify that your window frames are wood, so that's not a problem. However, modern insulated (double-glazed) windows may have metal as part of the seal on the glass (it would be visible looking in through the glass). If you have this, make sure the ladder line doesn't run parallel to the frame on the inside or outside.
Twisting your ladder line also helps reduce radiation and coupling to nearby metal.
The second issue is interference with the speakers and other electronic equipment. (Even if the effects are minor, you'll likely want to get them entirely cleaned up if you get into digital modes!) In priority:
Make sure that you have a suitable balun either built in to, or in addition to, your antenna tuner. (I've heard rumors that some tuners with balanced outputs have baluns that aren't actually very effective.)
Ground the antenna tuner: connect its ground terminal to the shortest path to earth ground you can get. If everything else is perfect, this is unnecessary, but it will help. (Note: All else being equal, making only this connection likely decreases lightning safety. Either do your research for a complete grounding system, or disconnect your antenna, and the additional ground, for any storm.)
Finally, if you can separate equipment, do so. (Move that USB hub!) Try to have one side of the room for your digital signals and the other for your RF signals.
Add ferrite chokes to all the cables in the room/house. This is a last resort because it is better to stop the signal getting out (of your feed line) than to stop it getting in (to everything else), but it may be necessary if you are getting interference not from your feed line but your antenna, i.e. the signal you're actually intending to transmit.